THE TIME RELATIONSHIPS BETWEEN APOPTOSIS OF NEURON AND ENDOTHELIOCYTE WITH THE EXPRESSION OF Bcl-2 AND Bax AFTER FOCAL CEREBRAL ISCHEMIA REPERFUSION IN RATS
- VernacularTitle:脑缺血再灌注后神经元和内皮细胞凋亡与Bcl-2和Bax表达的时相关系
- Author:
Yunliang GUO
;
Yingmao GAO
;
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Neuron;
Endothelial cell;
Protein expression,Bcl 2,Bax;
Cerebral ischemia,Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the time relationships between apoptosis of neurons and endotheliocytes with the expression of Bcl 2 and Bax after reperfusion of focal cerebral ischemia in rats. Methods Coronal sections of brain were analyzed using an in situ terminal deoxynucleotidyl transferase mediated biotinylated deoxyuridine triphosphate nick end labeling(TUNEL)and immunohistochemical staining methods to observe apoptosis of neurons and endotheliocytes,and expression of Bcl 2 and Bax after reperfusion(2,6,12,24hours and 2,3,7,14,21 days)of focal cerebral ischemia. Results 1.In the ischemic penumbra,apoptotic cells were increased at 2h after reperfusion,peaked at 12 24?h,then decreased successfully for 7 14?days.The time of apoptotic endothelial cells was 12?h later than that of apoptotic neurons.2.The expression of Bcl 2 protein began at 2?h after reperfusion,peaked at 12 24?h,and decreased for 7 14?days.3.Bax protein expressed from 6?h after reperfusion;peaked at 24 48?h,and lasted for 14days.4.The time phase of Bcl 2 expression was similar to the Bax is but later than it. Conclusion\ Apoptosis was a pattern of cell death after cerebral ischemia/reperfusion.The time of apoptotic endothelial cells was later than that of apoptotic neurons.Bcl 2 and Bax play a regulatory role in the apoptotic process.\;[
