Determination of liquiritin、liquiritigenin and iso-liquiritigenin in Licorice Mixture by HPLC
- VernacularTitle:HPLC双波长法测定复方甘草合剂中甘草苷、甘草素及异甘草素的含量
- Author:
Xiaoli ZHAO
;
Xiaobing CUI
;
Hao WU
;
- Publication Type:Journal Article
- Keywords:
HPLC;
Licorice Mixture;
dual wavelength;
liquiriitin;
liquiritigenin;
iso liquiritigenin
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a HPLC method for the determination of liquiritin、 liquiritigenin and iso liquiritigenin in Licorice Mixture.Methods: A Cromasil C 18 column was used. the mobile phase was methanol 0.5% acetic acid for gradient elution. Liquiritin、 liquiritigenin and iso liquiritigenin were determined by dual wavelength, ? 1=276 nm,? 2=372 nm.Results: The linear range of liquiritin was 50.0～1250.0ng, r= 0.9999; The average recovery was 98.30%, RSD =1.01%( n= 6). The linear range of liquiritigenin was 5.0～125.0 ng, r= 0.9998; The average recovery was 98.01%, RSD =0.68%( n= 6). The linear range of iso liquiritigenin was 3.0～6.0 ng, r= 0.9999. The average recovery was 98.37%, RSD= 0.89%( n= 6). Conclusion: This method is convenient, rapid and accurate. It can be used for quality control in production of Licorice Mixture.
