Determination of protopine and tetrahydropalmatine in Corydalis Decumbens (Thunb.) Pers. by HPLC
- VernacularTitle:HPLC测定夏天无中原阿片碱和延胡索乙素含量
- Author:
Xiaomeng LI
;
Ying LIU
;
Huawei LIAO
;
- Publication Type:Journal Article
- Keywords:
Corydalis decumbens (Thunb.) Pers;
protopine;
tetrahydropalmatine;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the content determination method of the main alkaloids protopine and tetrahydropalmatine in Corydalis decumbens (Thunb.) Pers. Methods: HPLC was used to determinate the main alkaloids protopine and tetrahydropalmatine in Corydalis decumbene (Thunb.) Pers. Supelcosil LC 18 DB Column was used, mobile phase consisted of Methol NaAc/HAc(75∶25 pH5.0) and detection wavelength at UV 285 nm. Results: Linearity of this method was good with the average recoveries, 98.04% and 99.63%. Conclusion: This method is accurate, reliable with good separability and reproducibility, it can be applied as standard for the control of Corydalis decumbens (Thunb.) Pers.
