Determination of scutellarin in BREVISCAPINE INJECTION by HPLC
- VernacularTitle:HPLC法测定灯盏花素注射液中野黄芩苷
- Author:
Shixuan ZHANG
;
Yujuan NIU
;
Haoran LV
;
Xiulan JU
;
- Publication Type:Journal Article
- Keywords:
HPLC Fingerprint;
BREVISCAPINE INJECTION;
scutellarin;
baicalin;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the determination method of scutellarin in BREVISCAPINE INJECTION using HPLC.Methods:The waters ODS C 18 column (5?m,3.9?150mm) was used. The methanol water glacial acetic acid (40∶60∶1) was used as a mobile phase. The detection wavelength of scutellarin was set at 335nm and internal standard baicalin at 280nm. The resolution of scutellarin's and baicalin peaks was at least 5. Number of theoretical plates of column is no less than 1000 to scutellarin. Results:The recovery of additive sample is 99.8%. RSD is no more 2.0%.Conclusion: It is generally in agreement with external standard method.
