Determination of conjugated bile acids in natural and artificial snake gall by HPLC
- VernacularTitle:HPLC检测蛇胆和人工蛇胆中结合胆汁酸的含量
- Author:
Hongtao XIAO
;
Guang YANG
;
Chaozhou SHI
;
- Publication Type:Journal Article
- Keywords:
HPLC;
artificial snake gall;
TCA;
TDCA
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine taurocholic acid (TCA) and taurodeoxycholic acid (TDCA) in artificial snake gall. Methods: HPLC was used in the quantitative analysis with spherisorb C 18 column, methyl alcohol 0.02 mol/L phosphric buffer (60∶40) as a mobile phase and detection wavelength at 210nm. Results: The linear range of TCA was from 0.28672 mg/mL to 2.8672 mg/mL, and the linear range of TDCA was from 0.26842 mg/mL to 2.6842 mg/mL. The average recoveries were 97.65% for TCA and 95.81% for TDCA. RSD were 0.79% and 3.27%, respectively. Conclusion: This method is simple and accurate.
