THE EFFECTS OF db-cAMP ON CaM LEVEL,PK Ⅱ ACTIVITY AND INHIBITION OF TRANSFORMED CELLS PROLIFERATION
- VernacularTitle:db-cAMP对转化细胞增殖抑制及其对CaM水平和PKⅡ活性的影响
- Author:
Tao ZHOU
;
Duanshun WANG
;
- Publication Type:Journal Article
- Keywords:
db cAMP;
Cells proliferation;
PKⅡ
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of the effects of db cAMP on the inhibition of cell proliferation and phenotypic changes in transformed cells. Methods The progression of cell cycle was analyzed by the flow cytometry.The transforming phenotype of C 3H 10 T 1/2 cells was observed by the soft agar colony formation.Northern blotting and radioimmunoassay were used to analyze the level of CaM expression.The activity of PKⅡ was determined through ? 32 p ATP incorporation assay. Results The proliferation of transformed C 3H 10 T 1/2 cells was inhibited markedly by 1mmol/L db cAMP.The db cAMP treated transformed cells were accumulated at G 1 phase.After treatment with db cAMP,the soft agar colony formation efficient in transformed cells was decreased evidently,simultaneously,cells altered in morphology,from rounded to flattened shape.In contrast to normal cells,CaM level and PKⅡ activity increased in transformed cells.However,both CaM level and PKⅡ activity in transformed cells treated by db cAMP decreased obviously.Conclusion\ The results indicate that not only alteration of CaM expression,but also the changes of the PKⅡ activity are involved in account for the cell transformation and induced differentiation of transformed cells.It is possible that the role for CaM in the cell transformation and induced differentiation may be mediatied by the effect of PKⅡ. \;[
