STUDY OF PROBE-PREPARATION OF THE EXPRESSION GENES FOR K562 CELL LINE
- VernacularTitle:K562细胞株表达基因探针制作的探讨
- Author:
Ji ZHU
;
Wenli MA
;
Ruhua YAO
;
Wenling ZHENG
;
- Publication Type:Journal Article
- Keywords:
Gene chips;
Gene probe;
Restriction display
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a better probe preparation method of gene chips for the further gene chips fabrication and its application in the gene expression. Method Using human leukemia K562 cells as material,applying a new kind of technology for gene differential display restriction display(RD PCR),we isolated a lot of cDNA fragments as probes of gene chips. Results This method has overcome the shortcoming of more pseudopositives in DD PCR,and was easy to be manipulated.Compared with the chip fabricated with full cDNA probes,the probe length is comparatively homogeneous,and the hybridization dynamics is apt to be controlled.Conclusion\ RD PCR technology could provide a new strategy for the probe preparation of gene chips.\;[
