HIGH LEVEL EXPRESSION AND ACTIVITY DETECTION OF SINGLE CHAIN IMMUNOTOXIN 183B_2ScFvPE38 AGAINST OVARIAN CARCINOMA
- VernacularTitle:抗卵巢癌单链免疫毒素183B_2ScFvPE38融合蛋白的高效表达及活性测定
- Author:
Fanglei YOU
;
Jie FENG
;
Yexia CHENG
;
Tianyun FU
;
Yu YAO
;
- Publication Type:Journal Article
- Keywords:
Single chain Fv antibody;
Immunotoxin;
Ovarian carcinoma;
Pseudomonas exotoxin;
High level expression
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the immunotoxin protein (183B 2ScFvPE38) which might be useful in immuno guided therapy for ovarian carcinoma and study the activity of the protein. Methods The methods of ELISA and cytotoxicity were used to study the immunotoxin after induced with IPTG and the activity of the immunotoxin. Results The expressed fusion proteins were detected mostly as inclusion bodies at high level, and soluble immunotoxins were also observed. The results showed liable activity of antibody part and toxic part. Conclusion The recombinant fusion protein 183B 2ScFvPE38 keeps the activity of both components and might be of great use in the future to deal with ovarian carcinoma. [
