Determination of Danshensu and Protocatechuic Aldehyde in Huoxue Mixture by HPLC
- VernacularTitle:HPLC测定活血合剂中丹参素、原儿茶醛的含量
- Author:
Jianzhong ZHANG
;
Qianzhou LU
;
Xinhong WANG
;
Rui AN
;
- Publication Type:Journal Article
- Keywords:
Huoxue mixture;
RP HPLC;
danshensu;
protocatechuic aldehyde
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the method of determination of the contents of Danshensu and Protocatechuic aldehyde in Huoxue Mixture simultaneously by RP HPLC.Methods:The determination was carried out with Inertsil ODS column. The mobile phase consisted of methanol 0.5% HAc (22∶78) and UV detection wavelength was 280nm. Results:The average recovery of standard addition were 96.7%, RSD =1.12% ( n =5) for Denshensu, and 97.0 %, RSD =1.53% ( n =5) for Protocatechuic aldehyde, There was a good linear relationship between the absorption area value and the concentration in the range of 1.25～10?g for Deshensu and 0.25?g～2.0?g for Protocatechuic aldehyde.Conclusion: The method is simple, acurate, reproducible and can be used for content determination of Huoxue Mixture.
