Determination of Hesperidin in Jinshuiliujun Decoction by RP-HPLC
- VernacularTitle:RP-HPLC法测定金水六君煎中橙皮苷的含量
- Author:
Shuhao GUO
;
Xiaogang HU
;
Yan BAI
;
Yingshen SHEN
;
Jie JIANG
;
Changyin ZHAO
;
- Publication Type:Journal Article
- Keywords:
hesperidin;
Jinhuiliujunjian;
RP HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The content of hesperidin in Jinshuiliujun Decoction (JSLJD) was determined by RP HPLC. Methods: The chromatographic column was Hyperisl BDS C 8(10?). The mobile phase was acetonitrile water containing 8% acetic acid (17∶83). The flow rate was 1.0 mL/min. The column temperature was 40 ?C and the detection wavelength was 284nm. The external standard and peak area method were used to determine hesperidin content. Results: There was a very good linear relationship between hesperidin and peak area when the sample injection was from 0.02 to 2.00?g. The average recovery of adding sample was 100.5% and RSD was 1.85%. Conclusions: This method is simple and believable. It can be effectively used for the quality control of JSLJD.
