Determination of glycyrrhizic acid in MAHUANG DECOCTION by HPLC when decocted separately or as a whole
- VernacularTitle:HPLC法测定麻黄汤分煎及合煎汤剂中甘草酸含量
- Author:
Peixue CAO
;
Guangyi LIANG
;
Bixue XU
;
Fengyun JIN
;
Zhuying HE
;
- Publication Type:Journal Article
- Keywords:
glycyrrhizic acid;
MAHUANG DECOCTION (Ephedra decoction);
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To determine the content of glycyrrihizic acid obtained when each individual ingredient in MAHUANG DECOCTION was decocted separately and then mixing the extracts with boiling water in comparison with that obtained by decocting the total composition together as a whole in the traditional way. Methods The contents of glycyrrhizic acid was determined by HPLC. Hypersil C 18 column was used, with acetonitrile∶0.1% acetic acid (33∶67) as the mobile phase and detected at the wavelength of 254 nm. Results The average recovery of glycyrrhizic acid when separately decocted was 102.43%, RSD=2.65%, while that of decoction in whole was 99.41%, RSD=3.11%. Conclusion The method was simple and accurate and was not interfered by other constituents in the prescription.
