Effect of Panax notoginseng saponins on myocardial Gs? mRNA expression of seriously scalded rat
- VernacularTitle:三七总皂苷对烫伤大鼠心肌Gs? mRNA表达的影响
- Author:
Haigang ZHANG
;
Xiaohui LI
;
Shuhui LI
;
Hongmei XU
;
Ya TAN
;
- Publication Type:Journal Article
- Keywords:
burn;
stimulatory G protein;
message RNA;
signal transduction;
Panax notoginseng saponins (PNS);
adenyl cyclase, cyclic AMP
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To investigate the effects of Panax notoginseng saponins (PNS) on myocardial Gs? mRNA expression of seriously scalded rat. Methods A 30% skin full thickness scald model was produced by immersing rat in 95 ℃ water for 10 s. The effects of PNS on myocardial Gs? mRNA level were observed with dot blotting hybridization and in situ hybridization technique; effects on cAMP and adenyl cyclase (AC) activities were determined with radioimmunoassay. Results Myocardial Gs? mRNA, AC activity and cAMP content were reduced significantly 3 h after scalding. PNS (100, 200 mg/kg) could markedly increase the level of myocardial Gs? mRNA expression. The elevated quantity was correlated markedly with PNS dosage (r = 0.95, P
