Application of gene technology in quality control of Chinese materia medica Ⅱ. Identification of Chinese Rhizoma Dioscoreae by DNA sequencing
- VernacularTitle:基因测序技术在中药质量研究中的应用(Ⅱ)——山药基原的DNA测序鉴别
- Author:
Yuping LIU
;
Baozuo HE
;
Hui CAO
;
- Publication Type:Journal Article
- Keywords:
Rhizoma Dioscoreae;
18S rRNA gene;
DNA sequencing;
origin identification
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To distinguish the genuine Dioscorea polystachya Turcz. from other species of Dioscorea L. such as D. persimilis Prain et Burkill, D. japonica Thunb., and D. alata L., conventionally used in some localities by sequencing their nuclear ribosomal RNA subunit (18S rRNA), to provide mole cular evidences for the quality control of drugs of Dioscorea L. Methods By direct PCR sequencing to detect the homology of 18S rRNA sequence of different species of Dioscorea L.. Results The 18S rRNA gene sequence of genuine D. polystachya, D. persimilis and D. japonica showed identical length of 1810 bp, whereas that of D. alata was 1807 bp. Multiple sequence alignment of 18S rRNA gene showed that the homology was identical between D.persimilis and the genuine D. polystachya; but a difference of 99.89% with D. japonica and 97.51% with D. alata. Conclusion DNA sequencing can provide an accurate and reliable mean for the identification of the origin and genuineness of Dioscorea L..
