Studies on establishment of calli culture for rapid propagation of Spiraea japonica
- VernacularTitle:药用植物粉花绣线菊的组织培养的建立与快速繁殖研究
- Author:
Yiming HU
;
Fanyuan GAN
;
Liping PENG
;
Xiaojiang HAO
;
- Publication Type:Journal Article
- Keywords:
Spiraea japonica L. f.;
cullus tissue;
rapid propagation
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To establish a calli culture system for the rapid propagation of Speiraea japonica L. f.. Methods Callus and shoot induction were carried out on MS, 6, 7 V or B 5 media with different parts of the plant such as stem tip, tender leaves and petiole as explants. Results A calli culture system was established for the rapid propagation of S. japonica. Conclusion MS cultural medium was found to be most suitable for calli induction. MS with 2.0 mg/L 2,4 D+0.3 mg/L KT can induce calli when the explants were used for the induction, with stem tips being the most satisfactory explant. Clusters of seedlings can be induced on MS+2 mg/L BA+0.1 mg/L NAA and when these seedlings were transferred to 1/2 MS +0.25 mg/L BA+0.5 mg/L NAA medium, root were developed to give young seedlings.
