Studies on chemical structure of polysaccharide Ⅰ obtained from Paecilomyces tenuipes
- VernacularTitle:细脚拟青霉多糖Ⅰ的化学结构
- Author:
Rong LU
;
Lisong SUN
;
Zhongfu WANG
;
Gengyuan TIAN
;
Yoshida TAKASHI
;
- Publication Type:Journal Article
- Keywords:
polysaccharide of Paecilomyces tenuipes Samson;
glucan;
methylation analysis
- From:
Chinese Traditional and Herbal Drugs
1994;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To study the isolation and purification of a polysaccharide, obtained from Paecilomyces tenuipes Samson, its molecular weight, sugar composition, and mode of linkage Methods Crude polysaccharide was extracted by water at ambient temperature and purified on Sephadex G 100 column Its monosaccharide composition was determined by ionic ion exchange column after complete hydrolysis with acid Their mode of linkage was determined by methylation and glycosidic linkage established by IR and NMR spectra Results HPLC spectrum showed that the polysaccharide was of homogeneous composition, which was also proved latter by GC MS and NMR Conclusion Polysaccharide obtained from P tenuipes Samson is ? (1→6) linked and composed of only D glucose The molecular weight was 2 05?10 4
