Determination of sarsasapogenin in crude Anemarrhena asphodeloides and its preparation by RP-HPLC-ELSD
- VernacularTitle:RP-HPLC-ELSD法测定知母药材及含知母制剂中菝葜皂苷元的含量
- Author:
Lan SHEN
;
Huping ZHU
;
Qi SONG
;
Ming CHEN
;
Guangping FAN
;
- Publication Type:Journal Article
- Keywords:
RP HPLC ELSD;
Anemarrhena asphadeloides Bunge;
sarsasapogenin
- From:
Chinese Traditional and Herbal Drugs
1994;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To show that ELSD is an excellent detector for the detection of chemical compounds devoid of chromophore, such as sarsasapogenin Methods Sarsasapogenin in crude Anemar rhena asphodeloides Bunge and its preparation was determinated by RP HPLC ELSD Results The well separated chromatographic peaks show linearity with recovery of the added sample of 100 5% in crude medicinal material and 91 38% in its preparation, r= 0 999 0 Conclusion The method was advanced, reliable, simple and can be used for quality control of crude A asphodeloides and its preparations
