Determination of dehydroxypresenegenin in Polygalae tenuifolia by HPLC
- VernacularTitle:HPLC法测定远志中去羟基远志皂苷元含量
- Author:
Youping LIU
;
Deguang WAN
;
Ying SONG
;
- Publication Type:Journal Article
- Keywords:
Polygalae tenuifolia Willd.;
dehydroxypresenegenin;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To establish an HPLC method for the determination of dehydroxypresenegenin for the quality control of Polygalae tenuifolia Willd. Methods Dehydroxypresenegenin was separated by ODS column (125 mm ? 4 mm, 5 ?m) with a mobile phase of methanol 0 05% phosphoric acid (74∶26) and detected at a wavelength of 210 nm. Results The calibration curve was linear in the range of 0 585～11 7 ?g,with a correlation coefficient of 0 999 99 . The average recovery was 99 21% (n=5), and RSD=0 25% (n=5). Conclusion The method is simple and accurate, with good repeatability and can be used for the quality control of Polygalae tenuifolia Willd and compound preparations of Radix Polygalae.
