Determination of osthol in LOTIO FRUCTUS CNIDII COMPOSITA by RP-HPLC
- VernacularTitle:复方蛇床子洗剂中蛇床子素的RP-HPLC测定
- Author:
Xinyu LIU
;
Fang WU
;
Guorong FAN
;
Jinhong HU
;
- Publication Type:Journal Article
- Keywords:
LOTIO FRUCTUS CNIDII COMPOSITA;
fruit of Cnidium monnieri (L.) Cuss.;
osthol;
RP HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To estabish a method for the quantitative analysis of osthol in LOTIO FRUCTUS CNIDII COMPOSITA by RP HPLC Methods Megestrol acetate was used as the internal standard Results Chromatographic separation of osthol and megestrol acetate was accomplished within 12 min on Hypersil ODS 2(200 mm? 4 0 mm, 5 ?m) column and acetonitrile 0 01 mol/L sodium dihydrogen phosphate (48∶52) as mobile phase The flow rate was 1 2 mL/min and the detection wavelength was 320 nm A good linearity was obtained in the range of 0 123 8～2 970 ?g/mL (r=0 999 6) for osthol and the average method recovery was (98 4?0 90) % with RSD=0 91% Conclusion The method was simple, rapid, accurate, reliable, and suitable for the quality control of LOTIO FRUCTUS CNIDII COMPOSITA
