Effect of serum Ruixiang Langdu (Stellera chamaejasme) extract on proliferation, clonal formation and DNA synthesis of mouse L_(1210) leukemic cells
- VernacularTitle:瑞香狼毒水提物小鼠药物血清对小鼠白血病L_(1210)细胞增殖、克隆形成和DNA合成的影响
- Author:
Zhengping JIA
;
Junjie FAN
;
Yanguang WANG
;
Jingwen XIE
;
Rong WANG
;
- Publication Type:Journal Article
- Keywords:
Ruixiang Langdu (Stellera chamaejasme L);
serum pharmacology;
L 1210 cells;
clone cells;
DNA synthesis
- From:
Chinese Traditional and Herbal Drugs
1994;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To explore, on the whole, the anticancer activity and mechanism of the aqueous extract of Ruixiang Langdu (Stellera chamaejasme L.) (SCLA). Methods Mice were given different i.g. doses of SCLA and their serum collected at different intervals after drug administration. The effects of the serum on the proliferation of mouse L 1210 leukemic cells were observed with MTT assay, clone formation and incorporation of [ 3H] TdR into DNA of the cells. Results Serum SCLA collected 1, 2, 4, 8 h after administration of 3, 6, and 12 g/kg SCLA showed significant decrease of MTT formazans and clone formation. Serum SCLA collected 2 h after drug administration showed more strong inhibition of cancer cell proliferation. It also inhibited the incorporation of [ 3H] TdR into DNA of L 1210 cells. Conclusion SCLA showed a remarkable inhibitory effect on proliferation and DNA synthesis of cancer cells cultured in vitro, which may be the main anticancer mechanisms of SCLA.
