Selection of Ginkgo biloba cell line for suspension culture and studies on stability of ginkgolide B in subculture
- VernacularTitle:银杏悬浮细胞系选育及继代培养中银杏内酯合成稳定性研究
- Author:
Jiajia LIU
;
Wenhui JIANG
;
Yong GUO
;
Suiping ZHENG
;
- Publication Type:Journal Article
- Keywords:
ginkgolide B (GB);
suspension cell lines;
subculture;
stability
- From:
Chinese Traditional and Herbal Drugs
1994;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To select the cell line of Ginkgo biloba L that may produce high yield of ginkgolide B (GB) in suspension culture and to study the stability of GB in subculture Methods Calculus was induced with stem, root and leaf of selected elite species and high yield suspension cell lines chosen by hypoxia stress Results Seven suspension cell lines with improved yield of GB were obtained Among which cell line MH 3 gave a 3 71 fold increase of cellular bioproduct with an increased content up to 302 ?g/g DW after culturing for 18 d, a leading record country wide In shaking bottle culture, it showed a consistent yield of GB with a content of 291 ?g/g DW in 6 successive subcultures, coefficient of variation=0 131 Conclusion The suspension cell line selected by hypoxia stress gave a higher yield and stability in successive transfer culture
