Purification of fibrinolytic enzyme from Perinereis aibuhitensis
- VernacularTitle:沙蚕纤溶酶的一种纯化方法
- Author:
Weiyun ZHANG
;
Ying CHEN
;
Shuijuan WANG
;
Zhonghao XIA
;
Renxiang TAN
;
- Publication Type:Journal Article
- Keywords:
Perinereis aibuhitensis Grube;
fibrinolytic enzyme;
isolation and purification
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To extract and purify a novel fibrinolytic enzyme from Perinereis aibuhitensis Grube Methods The enzyme was precipitated from the extract by ammonium sulfate, dialyzed, chromatographed on Sephadex G 100 column, and then purified by polyacrylamide gel electrophoresis Its fibrinolytic activity was assessed with fibrin plate method Results The purified enzyme showed an isoelectric point (PI) around 4 5 as tested by gel isoelectric focusing It consisted of two polypeptide chains with molecular weights around 33 000 u and 14 400 u, respectively Conclusion This was a novel fibrinolytic enzyme discovered from P aibuhitensis for the first time
