Isolation of active constituents in rhubarb by micellar electrokinetic capillary chromatography
- VernacularTitle:胶束电动毛细管色谱分离大黄中有效成分的研究
- Author:
Xiaoyu SHANG
;
Zhuobin YUAN
;
- Publication Type:Journal Article
- Keywords:
micellar electrokinetic capillary chromatography (MECC);
rhubarb;
anthraquinones
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To isolate and identify the four active constituents, chrysophanol, emodin, rhaponticin and rhein in rhubarb Methods Micellar electrokinetic capillary chromatography (MECC) with a buffer solution of 5 mmol/L H 3BO 3 NaOH containing 20 mmol/L of sodium deoxycholate (SDC) (pH=10) was used for their separation at a voltage of 17 kv Results The four active constituents were completely separated within 5 min Conclusion This method was found to be simple and rapid and gave satisfactory results
