Authentication of TCM Carapax Trionycis by allele-specific diagnostic polymeras chain reaction
- VernacularTitle:中药材鳖甲的位点特异性PCR鉴定研究
- Author:
Zhongquan LIU
;
Yiquan WANG
;
Kaiya ZHOU
;
- Publication Type:Journal Article
- Keywords:
Carapax Trionycis;
allele specific primer;
diagnostic polymerase chain reaction (PCR)
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Object\ To develop a convenient and practical method for the identification of Carapax Trionycis Methods\ Based on the sequence variations of 12S rRNA gene between Pelodiscus sinensis and other softshell turtles, a pair of allele specific primers was designed to distinguish P. sinensis from other species of Trionychidae. DNA were extracted and anplified and Carapax Trionycis could be identified accurately by polymerase chain reaction (PCR) using the primers Results\ Ten samples of turtle shell from different sources were indentified by the allele specific PCR with the primers The result indicated that three samples were substitutes of Carapax Trionycis, consilient with the result from DNA sequence analysis The mitochondrial 12S rRNA gene fragment of P. maculatus and a faked imitation had also been sequenced Conclusion\ The primers could be used as key components in Carapax Trionycis identification kit
