Effects of Zn~(2+) on cell cycle changes induced by multiglucosidum tripterygii
- VernacularTitle:锌离子与雷公藤多甙诱导的细胞生物周期变化
- Author:
Zhen′Gang WANG
;
Fang WANG
;
Aihua LIU
;
- Publication Type:Journal Article
- Keywords:
Multiglucosidum tripterygii;
Zinc;
Apoptosis
- From:
Chinese Journal of Rheumatology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Zn 2+ on human leukemia promyelocyte (HL 60)cycle changes induced by multiglucoside tripterygii (MT).Methods ① HL 60 phenotype and membrane protein Fas expression was detected.② HL 60 was cultured with MT observing the cell forms and cell cycle changes,DNA electrophoresis results and the effect of Zn 2+ on the cell cycle change.Results ① The HL 60 were CD4 +CD8 -CD19 -IFN ? +IL 8 -,and the Fas protein expression was poorer.② Following culture of HL 60 with MT,the apoptosis relative cell cycle change appeared in HL 60.This change mainly affected G 2/M and S stage cells,and presented dose time correlation.③ Zn + nearly all blooked the effect of MT induced HL 60 cell cycle change.Conclusion ① HL 60 has Th1 lymphocyte like characteristics,and the Fas protein expression is poorer.② Zn + affects the MT induced HL 60 cell cycle change.It suggests that Zn + might affect the therapeutic and side effects during clinical treatment of many autoimmune diseases with MT.
