Determination of Cinobufagin and Resibufogenin in Qianglijiuxin Dripping Pills by HPLC
- VernacularTitle:HPLC测定强力救心滴丸中华蟾蜍毒基和酯蟾毒配基的含量
- Author:
Xingping LUO
;
Lanxia WANG
;
Fen WANG
;
Bochong ZHANG
;
Ruichao LIN
;
- Publication Type:Journal Article
- Keywords:
Qianglijiuxin Dripping Pills;
Cinobufagin;
Resibufogenin;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the contents of cinobufagin and resibufogenin in Qianglijiuxin Dripping Pills. Methods: The HPLC method was used at Kromasil C 18 column of 250mm?4.6mm, 5?m, 0.5% Dipotassium Hydrogen Phosphate Acetonitrile (50∶50) as the mobile phase, 1.0mL/min as the flow rate at detection wavelength of 296nm. Results: For cinobufagin, the linear range was 0.67?g～4.7?g and the average recovery rate was 97.78% with RSD=1.2%. For resibufogenin, the linear range was 0.33?g～2.3?g and the average recovery was rate 99.61% with RSD=2.0%.Conclusion: The method can be applied to content determination of cinobafagin and Resibufogenin in Qianglijiuxin Dripping pills.
