Determination of Berberine Hydrochloride in Xiao A Lotion by HPLC
- VernacularTitle:HPLC测定消A洗液中盐酸小檗碱的含量
- Author:
Erli MA
;
Shen JI
;
- Publication Type:Journal Article
- Keywords:
Xiao A Lotion;
Berberine Hydrochloride;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a method to determine the content of a berberine hydrochloride in Xiao A Lotion. Methods: HPLC was used to determine the content of berberine hydrochloride in Xiao A Lotion. The separation was performed on Inertsil ODS 3 column with 0.05mol/L NaH 2PO 4 (pH3.0) acetonitrile (70∶30) as a mobile phase and the wavelength of UV detector was 270nm. Results: The Linearity of this method was well with the average recovery of 101.4%. Conclusion: The method is simple, reliable, and sensitive. It also shows good separating degree. It can be used in quality control of Xiao A Lotion.
