EFFECTS OF TADPOLE EXTRACT ON EXPRESSION OF THE RELATED ONCOGENES IN HL 60 CELLS
- VernacularTitle:蝌蚪提取液对HL-60细胞相关癌基因表达的影响
- Author:
Xiaoyu DU
;
Jingxiu BAI
;
Rong BAI
;
Xiaoli ZHANG
;
Baoying GU
;
- Publication Type:Journal Article
- Keywords:
The extract of tadpole;
Human promyelocytic leukemia (HL 60) cell line;
Differentiation;
Apoptosis.
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tadpole extract(T871 3) on tumor cells and its mechanism. Methods We studied the effects of T871 3 on proliferation, differentiation and apoptosis of HL 60 cells by cytomorphological observation, cytochemistry and TUNEL method. We also examined gene expression during the induction of apoptosis and differentiation in tadpole extract treated HL 60 cells by in situ hybridization and intact cell mRNA dot blot techniques. Results 1 T871 3 was able to inhibite HL 60 cells proliferation. 2 T871 3 was able to induce HL 60 cells to differentiate along monocyte macrophage lineage at low concentration, and apoptosis at higher concentration. 3 The differentiation of HL 60 cells was accompanied by downregulations of c myc,c myb gene expression, The apoptosis of HL 60 cells was accompanied by downregulations of c\|myc bcl 2 gene expression, suggesting that these genes may be involved in the apoptosis and differentiation process. Conclusion Tadpole extract may have effects on HL 60 cells through changing the oncogene expression. [
