Determination of flavonoids in Hypericum perforatum processed by different methods of drying by HPLC
- VernacularTitle:HPLC法测定不同干燥方法的贯叶连翘中黄酮类化合物的含量
- Author:
Qiuxia QIAN
;
Xiaodong CONG
;
- Publication Type:Journal Article
- Keywords:
HPLC;
Hypericum perforatum L;
rutin;
hyperin
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop a method for the determination of flavonoids in Hypericum perforatum L processed by different drying methods to provide a basis for the processing of the natural herbs Methods The chromatographic conditions were Discovery C 18 column (5 ?m, 4.6 mm ? 25 cm). detection wavelength 365 nm; mobile phrase : water : acetonitrile : phosphoric acid (825∶175∶1); flow rate 1.0 mL/min Results The contents of rutin and hyperin were at their maximum when dried at 60 ℃ for 4 h Rutin had a good linearity in the range of 0.107～2 675 ?g, average recovery rate 99 32%, RSD 1 007%, and hyperin in the range of 0 107～2 675 ?g, average recovery rate 99 54%, RSD 3 591% Conclusion Temperature is the main factor influencing the content of flavonoids The HPLC determination was shown to be rapid, reliable and simple, and may be used for the quality control of H perforatum
