Comparison on internal transcribed spacers (ITS) sequences of Tibetan medicine Saussurea medusa and its easily confusable species
- VernacularTitle:藏药雪莲原植物水母雪莲及其混淆种类的ITS序列比较和分子鉴定
- Author:
Jianquan LIU
;
Zhiduan CHEN
;
Anmin LU
;
- Publication Type:Journal Article
- Keywords:
Tibetan medicine;
Saussurea medusa Maxim;
internal transcribed spacers (ITS) sequences;
molecular authentication
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Object In order to identify the medicine at the molecular level, the internal transcribed spacers (ITS) of Saussurea medusa Maxim and its easily confusable species were sequenced Methods The double stranded DNA was amplified using PCR systems 9 600 kits and sequenced on an ABI 377 automated sequencer from both directions Results The ITS sequences of S medusa of different populations showed no variation, but there existed distinct variation between S medusa and its confusable species Conclusion ITS sequences can be used for the molecular authentication between S medusa and its confusable species
