Determination of Metamizole Sodium and Chlorphenamine Maleate in Zhongganling Tablets by Solid-Phase Extracting HPLC
- VernacularTitle:重感灵片中安乃近及马来酸氯苯那敏的固相萃取高效液相色谱法测定
- Author:
Yiying LIANG
;
Binghui ZHU
;
Huiwen LU
;
Jinxiong YU
;
Zhihua DENG
;
- Publication Type:Journal Article
- Keywords:
metamizole sodium;
chlorphenamine maleate;
zhongganling Tablets;
solid phase extraction;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for the determination of metamizole sodium and chlorphenamine malete in zhongganling Tablets. Methods: The sample was determined by ion pair HPLC after it was purified on Sep Pak C 18 microcolumn. The chromatographic conditions included: Hypersil DBS C 18 chromatographic column (250mm?4.6mm, i.d.5?m) as an anlaytical column, methanol mixed solution of sodium heptanesulfonate and glacial acetic acid (600∶400) as a mobile phase, the detection wavelength at 264nm and 1.0mL?min -1 of flow rate. Results: The average recoveries of metamizole sodium and chlorphenamine maleate were 99.6% (RSD was 2.1% and n was 6) and 98.0% (RSD was 1.5% and n was 6), respectively. Conclusion: Metamizole sodium and chlorphenamine maleate can be determined respectively by HPLC with the same mobile phase when Sep Pak C 18 microcolumn solid phase extraction method is used to substitute for the traditional sample pretreatment methods refluxing, extracting and concentrating, and sodium heptanesulfonate ion pair reagent in acid condition is selected.
