Determination of content of astragaloside A in Astragalus membranaceus powder forinjection by TLC-spectrophotometry
- VernacularTitle:薄层色谱-分光光度法测定黄芪粉针剂中黄芪甲苷的含量
- Author:
Chunxin YANG
;
Lixia SUN
;
Genying XU
;
- Publication Type:Journal Article
- Keywords:
Astragalus membranaceus (Fisch ) Bge powder for injection (AMPI);
TLC spectrophotometry;
astragaloside A
- From:
Chinese Traditional and Herbal Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop a method for the quality control of Astrgalus membranaceus (Fisch ) Bge powder for injection (AMPI) Methods TLC spectrophotometry was used to determine the content of astragaloside A in AMPI The mobile phase was chloroform methanol water (65∶30∶10) and detected at the wave length of 418 nm Results The regression equation within the spotted range of 80~240 ?g was A=1 538 C+ 0 189, with good linearity, r=0 999 8 The average recovery rate was 98 68% and RSD=2 52%(n=5) Conclusion The method used simple and easily available instruments and the rusults obtained were reliable and specific, suitable for the quality control of AMPI
