In-Vitro Culture and Rapid Propagation of Aloe vera L.Var.chinensis(Haw.)Berger
- VernacularTitle:中国芦荟离体培养和快速繁殖
- Author:
Hong HE
;
Chunlai LIU
;
Sheng'E XIAO
;
Honghua XU
;
Binbin FANG
;
Liyi LIANG
;
- Publication Type:Journal Article
- Keywords:
ALOE/growth;
IN VITRO CULTURE;
CULTURE MEDIA/standards
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find out technological evidence for the rapid propagation of Aloe vera L.var.chinensis (Haw.)Berber. Methods The technique of plant tissue culture was used to study the rapid propagation of Aloe vera L.Var.Chinesis(Haw.)Berger.The shoot growing from the underground stem of Aloe vera L.Var.chinensis(Haw.)Berger was taken as the explant to induce fascicular bud. Results The optimal culture medium was MT.MT culture medium added with 6-benzylaminopurine(BA) was effective to induce the shoot formation and increase the direct shooting rate.The optimal BA concentration for the induction and formation of shoot was 2 mg?L -1 ,the shooting rate being 100%.As for the induction of rooting,MT culture medium adding with 0.5 mg?L -1 naphthalene acid was optimal,the rooting rate being 100%. Conclusion The culture of Aloe shoot growing from the underground stem,proper culture medium and BA concentration are important for the rapid propagation of Aloe.