Determination of Paeoniflorin and Glycyrrhizic Acid in Qianggan Capsules by Dual Wavelength RP HPLC
- VernacularTitle:RP-HPLC测定强肝胶囊中芍药苷及甘草酸的含量
- Author:
Jiangfeng YANG
;
Li CHEN
;
Jingxia SUN
;
- Publication Type:Journal Article
- Keywords:
paeoniflorin;
glycyrrhizc acid;
RP HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the contents of paeoniflorin and glycyrrhizic acid in Gianggan Capsules by RP HPLC. Methods: The Chromatographic method was carried out on Kromasil C 18 column using acetonitrile phosphate buffer solution (pH=5) (20∶80) as a mobile phase. The detection wavelength was set at 230nm and 250nm. The flow rate was 1ml/min. The column temperature was 40℃. Results: The paeoniflorin and glycyrrhizic acid calibration curves were linear at the ranges of 0.48～4.80?g and 1.08～10.81?g(r=0.9999). The average recoveries were 100.5% with RSD 1.2% for paeoniflorin and 98.9% with RSD 0.9% for glycyrrhizic acid. Conclusion: This method is simple, reliable and provides a reference standard for the quality control of Gianggan Capsules.
