RAPD Differentiation of wild Ginseng Species
- VernacularTitle:用随机扩增多态DNA(RAPD)技术鉴定野山人参
- Author:
Jianmi DING
;
Shuwen WAN
;
Qichun MEI
;
Yongping SHAO
;
- Publication Type:Journal Article
- Keywords:
RAPD;
wild Ginseng;
cultivated Ginseng
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the potentiality of random amplified polymorphic DNA (RAPD) technique for the differntiation of wild and cultivated Ginseng.Methods: DNA templated were extracted from wild and cultivated Ginseng of the crude drugs that were identified by morphology, and RAPD technique was applied to produce electrophoresis pattern. Results: Among the 80 primers screened, one was able to generate reproducible band, characteristic RAPD marker band which was unique to each of wild Ginseng samples was identified. Conclusion: The results demonstrated that RAPD marker technique could effectively authenicate wild and cultivated Ginseng samples.