Purification of Polysaccharide Peptide of Coriolus Versicolor in Affinity Chromatography
- VernacularTitle:亲和层析纯化云芝糖肽
- Author:
Yinlin LEI
;
Xiaodong WANG
;
Zuozhen LIU
;
Xingyan WU
;
- Publication Type:Journal Article
- Keywords:
Polysaccharide peptide of Coriolus versicolor;
Affinity Chromatography;
Separation and purification
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study a new purification method of Polysaccharide Peptide of Coriolus versicolor in affinity chromatography. Methods: Isotherm and kinetics in absorption and the optimal conditions of absorption and elution were studied through static experiments. The static absorption capacity q m and absorption constant K d were calculated according to Chase model. Results: q m =55.57mg/g wet resin, K d =5.312g/L, the dynamic absorption capacity is 43.1mg, polysaccharide/g wet resin and 10.3mg protein/g wet resin.Conclusion: Affinity chromatography can be used to purify PSP preliminarily.
