In-Vitro Culture and Plant Regeneration of Morinda officinalis How
- VernacularTitle:巴戟天离体培养及植株再生的研究
- Author:
Hong HE
;
Sheng'E XIAO
;
Jianchun XIAN
;
Honghua XU
;
- Publication Type:Journal Article
- Keywords:
MORINDA OFFICINALIS/growth and development;
@IN-VITRO CULTURE;
@PLANT REGENERATION
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The sterile stem tips, nodular and non-nodular stem of Morinda officinalis How were cultured in vitro. The results showed that the most suitable procedure for sterilization of explants was soaking into 0.1% HgCl 2 solution for 10 to 15 minutes after pretreating with 70% ethanol for 30 seconds. MT culture medium with BA was effective to induce direct shooting of stem tips and nodular stem, the shooting rate of nodular stem being 97.5% . The optimal BA concentration was 1 mg?L -1 , the shooting rate would decrease when the concentration of BA increased. As for the induction of rooting, MT culture medium adding with 0.2 to 0.5 mg?L -1 NAA was optimal, the rooting rate being over 80.0% .
