IIsolation and culture of enterochromaffin like cell and its functionaldetermination
- VernacularTitle:胃内肠嗜铬样细胞的分离与培养及功能测定
- Author:
Kun MIU
;
Liang SUN
;
Lh TANG
;
ETAL
;
- Publication Type:Journal Article
- Keywords:
Enterochromaffin like cell;
Isolation;
Culture
- From:
Chinese Journal of Digestion
1998;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
95 %viability .Histaminesecretionismeasuredbyenzymeimmunoassay .Basalsecretionwas (0 .6 ? 0 .2 )nmol/ 10 3cell.GastrinstimulatedhistaminesecretionwithEC50 of 3? 10 -10 mol/Landwasinhibited bypretreatmentofgastrinreceptorantagonistL3652 66,(IC50 10 -8mol/L) .TheDNAsynthesisofECLcell wasdeterminedbyBrdU .GastrinstimulatedECLcellDNAsynthesiswithEC50 of 2? 10 -11mol/L . ConclusionAboveresultsindicatedthatECLcellcouldlivewellinculturesystemandprovideagoodmeansfor investigatingcentralroleofECLcellfunction .
