DETECT mRNAs ENCODING PREPROENKEPHALIN A IN THE ADRENAL GLANDS USING THE TECHNIQUE OF IN SITU HYBRIDIZATION HISTOCHEMISTRY
- VernacularTitle:用原位杂交组化方法探测肾上腺中合成前脑啡肽原的mRNA
- Author:
Chengsi YU
;
Zhenshan LU
;
Kequn OU
;
- Publication Type:Journal Article
- Keywords:
Adrenal gland;
mRNA encoding preproenkephalin A;
in situ hybridization histochemistry
- From:
Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
cDNA probes encoding preproenkephalin A (PPA) were used to detect the mRNA codings within the sections of adrenal glands of guinea pigs, processing according to the following procedures; perfused the animals with Zambonis' or 4% paraformaldehyde fixative; rinsed the block of adrenal glands for 12 hours in O.1mol/L phosphate sodium solution; the block were cut at -15℃ in a cryostat; Then the sections were prehybridized and hybridized with the biotinylated cDNA encoding PPA. The sections were incubated in the solution containing streptavi- din-biotinylated HRP and chromostained with DAB. The positive reactive produ- cts appeared in the cytoplasm of about 90% of adrenal medullary cells.
