AN OBSERVATION OF EXPRESSION OF Ia-ANTIGEN ON CELLS IN SUSPENSION FROM UNSTIMULATED RAT SPLEENS
- VernacularTitle:未受刺激的大鼠脾悬液细胞的Ia抗原表达的观察
- Author:
Ning YANG
;
Jiadi XU
;
Jinkun CHANG
;
Baoxing CHA
;
Lin YUAN
;
- Publication Type:Journal Article
- Keywords:
Ia-antigen;
Lymphoid dendritic cell;
Macrophage;
Lymphocyte;
Rat spleen
- From:
Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Expression of Ia-antigen on cells in lymphoid dendritic cells (LDCs)-enrt- ched fractions isolated from unstimulated rat spleens was observed with ABC immunoperoxidase and indirect immunofluorescent staining methods. The results appeared that there were three types of cells in stained preparaions: 1. Large cells with dendritic processes on their surfaces showed dark brown in colour with ABC staining method and emitted intense light-green fluorescence with immuno- fluorescent staining technic. 2. Large rounded or ovoid cells without obviously ramifications revealed colourless or light yellow-brown in colour with ABC sta- ining method and emitted no or weak yellow-green fluorescence with immuno- fluorescent staining technic. 3. Spherical lymphocytes of varying in size were similar to type 2 cells in immunoreactivity. The results of SEM and S-100 pro- tein staining confirmed that these three types of cells were LDCs, macrophages and lymphocytes in turn. It is indicated that LDCs consistently expressed Ia- antigen in large amount on their surfaces.
