IMMUNOHISTOCHEMICAL STUDY OF POSTNATAL DEVELOPMENT OF GNRH NEURONS IN THE RAT BRAIN
- VernacularTitle:大鼠脑内GnRH神经元生后发育的免疫组织化学研究
- Author:
Tianbao SONG
;
Qianwu LI
;
- Publication Type:Journal Article
- Keywords:
GnRH neurons;
Postnatal development;
Immunohistochemistry;
Image analysis;
Rat
- From:
Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
The morphology of the GnRH neurons in the rat brain during postnatal development was studied quantitatively using the ABC immunostaining method and an image analyser. The pattern of distribution of the GnRH neurons in different ages animals used was similar. GnRH cells of rats aged 1-7 days were small in size with short processes, pale-stained, and smooth in outline The number of GnRH cells in 1 day rats was not significantly different from that of adult, but a significant decrease in the GnRH cell number appeared in 7 day rats. In postnatal day 14, Gn-RH neurons reached adult level in cell body size, number and staining intensity. The GnRH fibers and terminals in the median eminence were gradually increased and reached adult level up to 14 postnatal days. These results suggested that the number of GnRH neurons is determined shortly after birth; the second postnatal week is the critical period for the development of GnRH neuronal morphology; and the establishment of function of the GnRH neuronal system may be earlier than its morphological maturation.
