IMMUNOHISTOCHEMICAL STUDY ON THE DISTRIBUTION OF von WILLEBRAND FACTOR
- VernacularTitle:Von Willebrand因子分布的免疫组织化学研究
- Author:
Nailin LI
;
Jin LI
;
- Publication Type:Journal Article
- Keywords:
von Willebrand factor;
Endothelium;
subendothelium;
Platelet;
Immunocolloidal gold-silver technique;
ABC technique
- From:Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
By using immunocolloidal gold-silver technique and ABC technique, an antihuman von Willebrand factor (vWF) monoclonal antibody, SZ-29, was applied to study the distribution of vWF in vessels and platelets. The results demonstrated that vWF distributed in the endothelium, basal membrane, subendothelium and media near internal elastic membrane of abdominal aorta, umbilical artery and umbilical vein. And the densest vWF-specific staining was found in the endothelium and its basal membrane. At the microscopical level, we found that vWF-specific staining was granule--like and scattered in most platelets, but about 3-5% of the platelets were found entirely silver-stained. At the ultrastructural level, we found that vWF was stored in ?-granules and there was little platelet membrane-bound vWF in resting platelets. However, in stimulated platelets, plenty of membrane-bound vWF was found on the platelet surface and open canalicular system membrane besides the vWF-specific staining in ?-granules. And vWF-specific staining might be found in platelet pseudopodia, especially at their tips,
