A QUICK DETECTION OF MYCOPLASMA IN CONTAMINATED-CELL CULTURE BY DAPI STAINING METHOD
- VernacularTitle:DAPI染色法快速检测细胞培养中支原体的污染
- Author:
Xing ZENG
;
Zhaocong CHEN
;
- Publication Type:Journal Article
- Keywords:
Mycoplasma;
Cell culture;
DAPI
- From:
Acta Anatomica Sinica
1989;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
With the method of specific binding of DAPI and DNA, the mycoplasma contamination cell cultures are sensitively detected. In this way, the monolayer cells can be determinated if a certain cell culture has been contaminated by some mycoplasma. The process is simple and can be done within thirty minutes. This method can be widely applied as a routine determination for detecting mycoplasma contamination in laboratory cell cultures.
