VARIATION IN LOCALIZATION OF CONCANAVALIN A RECEPTORS ON GASTRIC CANCER CELL LINE AND FIBROBLASTS
- VernacularTitle:人胃癌细胞系和成纤维细胞的 Con A 受体分布的变异
- Author:
Jiacun FANG
;
Minying NIU
;
Yongjin SHI
;
Daishu WANG
;
Menglian ZHAO
;
- Publication Type:Journal Article
- Keywords:
Concanavalin A receptor;
Gastric cancer cell line;
Fibroblast
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The distribution of receptor sites for concanavalin A (Con A) on the cellsurface of human gastric cancer cell line (MGC 80-3) and fibroblast cell cultures wasstudied with electron and light microscope after application of Con A-peroxidasemethod.The cytochemical reaction of the majority of non-prefixed MGC 80-3 cellsshows a striking tendency to be more uneven distribution than on fibroblasts.Clustering,patching and capping of Con A binding sites on the cancer cells anduniform distribution on the fibroblasts were observed.The effects of incubationtemperature,prefix treatment,different phase of cell cycles and cell growth condi-tions on the distribution of Con A receptor complexes were observed.The possiblereasons for the more irregular distribution of the cytochemical reaction product onthe MGC 80-3 cells than the fibroblasts are discussed.
