OBSERVATIONS ON THE MORPHOLOGICAL CHANGES OF EPIDERMAL LANGERHANS CELLS IN REPEATED SKIN IRRITATION TEST WITH BENZALKONIUM BROMIDE AND DINITROCHLOROBENZENE
- VernacularTitle:新洁而灭和二硝基氯苯多次刺激皮肤后郎格罕细胞形态变化的动态观察
- Author:
Jialin FANG
;
Jihe LIU
;
Huizhen SUN
;
Jingzhong LIU
;
Hongzhi GUO
;
Kexian GU
;
- Publication Type:Journal Article
- Keywords:
Morphological changes;
Epidermal Langerhans cells;
Benzalkonium bromide;
Dinitrochlorobenzene;
Enzyme histochemistry;
Guinea pig
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The kinetic changes in morphology and cellular density of epidermal Langerhanscells (LC) in the guinea pig were observed by ATPase cytochemical staining techniqueafter repeated skin application of 5%,3% and 1% of benzalkonium bromide (BB,primary irritant) and 0.05% dinitrochlorobenzene (DNCB,allergen) respectively.We have found that there was a significant difference in the density and morpho-logical change of LC between BB and DNCB application.Treatment with 5% BBcould induce a reversible decline in LC density and changes in cell processes,andwith 0.05% DNCB,the number of LC was decreased and the ATPase activity wasweakened only in the late stage of treatment.The significance of using the kineticchanges in morphology and cellular density of LC as the criteria for the safetyevaluation of weak allergens and weak primary irritants as well as cosmetics wasdiscussed.
