MALIGNANT PHENOTYPE ANALYSIS OF HYBRIDS FROM THE FUSION OF HUMAN PROMYELOCYTE LEUKEMIA CELLS AND MOUSE BONE MARROW NUCLEATED RED CELLS
- VernacularTitle:人早幼粒白血病细胞与小鼠骨髓有核红细胞杂交后恶性表型分析
- Author:
Youhua LIU
;
Shepu XUE
;
- Publication Type:Journal Article
- Keywords:
Malignant phenotype;
Oncogene;
Human leukemia cells;
Mouse nucleated red cells;
Cell hybridization
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The heterospecies hybrid cells(HL-N)from the fusion of human promyelocy-tic leukemia mutant cells(HL-60-AR)and mouse bone marrow nucleated red cellswere established in HAT selective medium.Malignant phenotype comparative analy-sis between parental tumor cells and hybrid cells showed that growth ability ofhybrid cells was decreased.The hybrid cells reduced their DNA synthesis rate andlost the ability of colony-forming in 0.3% soft agar medium.The cells lost tumor-producing ability when they were transplanted into nude mice also.Inhibition orreduction of c-myc oncogene expression was demonstrated by Northern molecularhybridization techniques.The ultrastructure of hybrid cells were also different fromtheir parental cells.These results mentioned above showed that the mouse bone mar-row nucleated red cells might provide some peculiar factors(both nuclear factorsand cytoplasmic factors)to inhibit the expression of HL-60-AR cell malignant phe-notypes.
