HISTOCHEMICAL STUDIES OF THE EFFECT OF HIGH DOSAGE LUTEINIZING HORMONE RELEASING HORMONE ANALOGUE(LH-RH-A)ON THE ENDOMETRIUM IN PSEUDOPREGNANT RATS
- VernacularTitle:大剂量促黄体生成素释放激素类似物对假孕大鼠子宫内膜影响的组织化学研究
- Author:
Jing HE
;
Maowei WANG
;
- Publication Type:Journal Article
- Keywords:
Histochemistry;
Decidual cell and stromal cell of uterus;
Luteinizing hormone releasing hormone analogue;
Pseudopregnancy;
Rats
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
29 virgin female Wistar rats were divided into experimental and control groupson the 5th day of pseudopregnancy.The experimental animals were injected with200 ?g.of LH-RH-A subcutaneously daily in 5th-8th day,while in the controlgroup physiological saline was used instead.Both experimental and control animalswere killed on 9th,11th,13th days of pseudopregnancy.Histochemical techniques were applied to cryostat sections to show the activityof the following enzymes:SDH,LDH,NE,~5△A-3?-HSD,G6Pase,TPPase,AlPand AcP;PAS,Oil red O,and gallocyanin-chromalum staining methods were usedto show carbohydrates,fats and RNA respectively.The above enzymatic reactions of the decidual cells of the experimental animalswere negative,while those of the stromal cells were positive on 9th day.Incomparison,the results of the decidua of the control group on 13th day are verysimilar to the decidua of the experimental group on the 9th day.The resultssuggest that a high dosage of LH-RH-A only advances degeneration of thedecidua.
