APPLICATION OF FOUR KINDS OF ELECTRON MICROSCOPIC TECHNIQUES TO HL-60-AR CELL ULTRASTRUCTURAL STUDY
- VernacularTitle:4种电镜技术在 HL-60-AR 细胞超微结构研究中的应用
- Author:
Youhua LIU
;
Yunqing WANG
;
Qiwu LU
;
Peirong ZHAO
;
Shepu XUE
;
- Publication Type:Journal Article
- Keywords:
Ultrastructure;
Acid phosphatase;
HL-60-AR cells;
Retinoic acid;
Scanning electron microscope;
Transmission electron microscope;
Freeze etching
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The fine ultrastructure and localization of acid phosphatase in cell ultrastructuralevel of a HGPRT-human promyelocytic leukemia cell mutant(HL-60-AR)arestudied by scanning electron microscopy,transmission electron microscopy,freezeetching,and electron microscopic cytochemistry techniques.The results of theobservation show that the ultrastructural characteristics of HL-60-AR cells aresimilar to that of HL-60 cells.There are microvilli and ridges over cell surface.Thecells have large nucleus with prominent nucleoli,and numerous nuclear pores.Thereare less developed Golgi complex,expanded rough endoplasmic reticulum andabundant polyribosomes.After treatment with retinoic acid(RA)at 10~(-6) mol/L for 5days,HL-60-AR cells differentiate along myeloid pathway and have a decreasednucleocytoplasmic ratio accompanied with nuclear condensation and segmention.A (?)ignificant increase of specific granules is demonstrated.Microvilli of the cellsdisappear,surface features of the treated cells become more irregular and largeprotrusion and blunt pseudopodia appear.Increase of acid phosphatase content localizedon azurophilic granules(lysosomes)and Golgi complex is showed.The application offour kinds of electron microscopic techniques might provide the best way foridentifying cell ultrastructure.
