THE EFFECT OF GOSSYPOL ACETATE ON ULTRASTRUCTURE OF HUMAN SPERMATOZOA STUDIED BY FREEZE-REPLICA
- VernacularTitle:棉酚对人类精子作用的冷冻复型法超微结构研究
- Author:
Jianzhang LEI
;
Guohua YING
;
Lansun HEI
;
Xiangyin LI
;
Yafei WANG
;
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to elucidate the ultrastructural changes of human spermatozoa afteradministration of gossypol acetate 40 mg/day for 50 days,freeze-replica techniqueswere employed.In addition to the ordinary changes of spermatozoa,such as swelling,wasting,atrophy,tortuosity,nodulation in the head and/or tail region and fracture betweenhead and tail,the internal structure,biomembrane and intramembranous particles ofsperm also showed relatively more sensitive changes.The changes of biomembranerevealed certain shift in regard to the firmness of reciprocating attachment of inner-and outer-leaflets of the unit membrane.On the other hand,the changes of intra-membranous particles revealed a specialized arrangement,this is,on the plasmamembrane of head and tail they were concentrated into lattice work,whereas,in thehind-head region they accumulated as clusters.In the hind-acrosomal membranetwo types of particles,large and small,may be distinguished,the smaller ones werearranged orderly in lines of all directions.As for the nuclear pores,no changewas found in this study.It is considered that the influences of gossypol upon human spermatozoa,firstof all,were shown in the biomembrane and intramembranous particles,and theordinary changes in appearance were the secondary result.In the discussion,it'ssuggested that a special agent,gossypol receptor,responsible for the gossypol effectsto the sperm,may be present in the plasma membrane of the spermatozoa.
