THE SEGMENTAL DISTRIBUTION OF THE LABELLED SPINAL GANGLIONIC CELLS AFTER HRP INJECTION INTO THE COELIAC GANGLION OF THE CAT
- VernacularTitle:猫腹腔神经节注射HRP后脊神经节标记细胞的节段分布
- Author:
Fu DU
;
Enhua YU
;
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Nine healthy adult cats were used in the investigation.Lumbar incision wasmade in one side and the coeliac ganglion was exposed under Nembutal anesthesia.One or two mg of HRP(Sigma Type Ⅵ)dissolved in 7 to 18 microlitres of distilled water was slowly injected into the ganglion via two or three insertions.Theleft coeliac ganglion was injected in four cats,the right in the other four,and theganglia of both sides were injected in the rest one.After a postoperative survivalof 4～5 days,the animals were perfused through the heart with 2% paraformalde-hyde and 1.25% glutaraldehyde in 0.1 M phosphate buffer(pH 7.4).Frozen serialsections(48 micra thick)of the postfixed spinal ganglia(C_3～L_7)were made andprocessed for the blue reaction using benzidine according to the Mesulam's eighthprocedure.The sections were counterstained with neutral red.HRP granules in the labelled cells in the spinal ganglia were discrete and easilyidentified under bright-field microscope,although the number of the granules variedfrom cell to cell.After the injection into the coeliac ganglia in ten sides,a totalnumber of 12,381 labelled cells were observed in the spinal ganglia.The greatermajority of them(more than 99%)were found ipsilateral to the side of injection.The labelled cells were found in the spinal ganglia from T_2 to L_2,but most of thesecells(78.1%)were distributed in the T_5 to T_9 segments.Furthermore,56.26% ofthe total number of them was situated at the level of T_(6～8),with the highest densityof the labelled cells in the seventh thoracic ganglion,constituting 22.19% of thetotal number.Although the number of labelled cells observed varied from case tocase,their percentage in the constitution of each ganglion in every case was similarto the general plan given above.There is no conspicuous difference between theleft and right sides in respect to the segmental distribution of labelled cells.Thelabelled cells were mainly of medium and small sizes.The larger cells,whichaccount for only 2.78% of all labelled cells,were very rare,and most of themwere found in the T_(5～9)spinal ganglia.The results of our investigation show that the visceral sensory fibers passingthrough the coeliac ganglion in one side come mostly from the ipsilateral spinalganglia T_5 to T_9.And about half of these fibers originate from T_6 to T_8 ganglia.Considering the fact that the greater majority of labelled cell bodies are of mediumor small size,it has been inferred that the fibers that arise from them are finemyelinated or unmyelinated and a part of them,at least,mediate the visceral pain.The labelled larger cells might be related to Pacinian corpuscles in the mesentery.It is difficult for the superior mesenteric ganglion to be separated from the coeliacganglion in cat;therefore,the labelled sensory neurons described above include,at least,a certain number of cell bodies of afferent fibers which traverse thesuperior mesenteric ganglion.
