MORPHOLOGIC STUDIES ON THE AGEING CHANGES IN CENTRAL NERVOUS SYSTEM OF RAT Ⅰ.CELL LOSS IN CEREBRAL AND CEREBELLAR CORTICES
- VernacularTitle:大鼠中枢神经系统衰老的形态学研究——大脑皮质与小脑皮质的神经元消逝
- Author:
Jiaji HOU
;
Zhixun LIU
;
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to examine whether the neuronal losses occur in cerebral and cerebellarcortices,we used two age groups of rats,the adult group aged 12 months containing8 animals and the old group aged 23～24 months,10 animals.After intraventriculardouble infusions with Koenig's acacia gum-formalin,a small piece of brain tissue,lessthan 2 mm in thickness,were removed from the cerebral and cerebellar cortices ofeach animal.All tissues were sectioned in paraffin and stained by H and E.Thecells were counted directly with an occular micrometer,in an area of 200 ?m~2 incerebral cortex and in a straight line of a length span of 5 mm in cerebellar cortexrespectively.The data from two age-groups were treated statistically.A significant neuronal loss were observed in both of cerebral and cerebellarcortices in the old rats.The pyramidal cells of V layer of parietal area fell from themean value of 22.75?1.76/200 ?m~2 in the adult group to 16.68?1.99/200 ?m~2 in theold.The decrease of percentage was 26.7%.The Purkinje cells fell from 98.87?3.72/5mm in the adult to 77.2?8.27/5 mm in the old,and the decrease of percen-tage was 21.9%.The t-test demonstrated that the difference between two age-groups both in cerebral and cerebellar cortices were significant(P
